Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: IHC staining of mp53 in SCCHN samples shows mainly nuclear and some cytoplasmic expression patterns. IHC staining of FGFR3 shows membrane and cytoplasmic expression. Four representative images are presented: mp53+/FGFR3+, mp53+/FGFR3-, mp53-/FGFR3+, mp53-/FGFR3- and negative control. (200 x magnification).

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques: Immunohistochemistry, Expressing, Membrane, Negative Control

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Univariate association of FGFR3 with clinical characteristics in cohort 1.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: FGFR3 association with clinical characteristics in cohort 2.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Correlation of FGFR3 with p53 and mp53 in cohort 1.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Pearson correlation coefficients (p-values) between FGFR3 and mp53, cytoplasmic and nuclear mp53 when p16 is negative.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Pearson correlation coefficients (p-values) between FGFR3 and mp53, cytoplasmic and nuclear mp53 when p16 is positive.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Cohort 1, univariate analysis of overall survival.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Cohort 1, univariate analysis of disease free survival.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Multivariate survival analysis on disease free survival.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: KM plots were generated using median weighted index (WI) of mp53 (3.5) and FGFR3 (35) as the cut off values (>median as positive and ≤ median as negative). High FGFR3 and mp53 levels correlated with worse OS (A) and DFS (B).

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques: Generated

Journal: Cells

Article Title: Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury

doi: 10.3390/cells10112998

Figure Lengend Snippet: Doxorubicin increases FGF-1 and PDGF-B protein levels in adult heart ventricular cells. Mice were given intraperitoneal injections of saline (S) or doxorubicin (D) and their hearts were harvested after 24 h (Day 1) or 72 h (Day 3). Using Western blotting, lysates containing equal amounts of total protein from the ventricular tissue of the differently treated animals were tested for FGF-1 and PDGF-B protein levels. When standardized against GAPDH, the level of both proteins (FGF-1 and PDGF-B) was found to be significantly higher in the Dox-treated ventricles than in the saline treated ventricles by Day 3. * p < 0.05 vs. saline (S), One-way ANOVA with Tukey’s multiple comparisons test, N = 3 independent experiments for each group.

Article Snippet: For the analysis of growth factor effects, the wells contained 1% FBS (Low Serum) DMEM and 40 ng/mL of FGF-1 (rMuFGF1 TM , Gold Biotechnology, St. Louis, MO, USA), its neutralizing antibody (Santa Cruz sc-7910, 100 ng/mL), or both.

Techniques: Western Blot

Journal: Cells

Article Title: Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury

doi: 10.3390/cells10112998

Figure Lengend Snippet: FGF-1 and PDGF-B increase embryonic ventricular cell migration, whereas their neutralizing antibodies decrease migration. 300 μL of 0.5 × 10 6 cells/mL serum-free cell suspensions were incubated for 22 h at 37 °C in polycarbonate membrane inserts with 8 μm pores, with surrounding wells containing 1% FBS DMEM and FGF-1, its antibody, or both ( A ) or PDGF-B, its antibody, or both ( B ). The inserts were then removed, migratory cells that had passed through the pores were washed and stained, and the cell stain was solubilized for measurement of absorbance. * p < 0.05 vs. all other groups; # p < 0.05 vs. all other groups, One-way ANOVA with Tukey’s multiple comparisons test, N = 3 independent experiments for each group.

Article Snippet: For the analysis of growth factor effects, the wells contained 1% FBS (Low Serum) DMEM and 40 ng/mL of FGF-1 (rMuFGF1 TM , Gold Biotechnology, St. Louis, MO, USA), its neutralizing antibody (Santa Cruz sc-7910, 100 ng/mL), or both.

Techniques: Migration, Incubation, Staining

Journal: Journal of cell communication and signaling

Article Title: Dysregulation of Notch-FGF signaling axis in germ cells results in cystic dilation of the rete testis in mice.

doi: 10.1007/s12079-021-00628-0

Figure Lengend Snippet: Fig. 3 Inhibition of FGF signaling results in dilation of the rete testis (RT) in neonatal testicular explants. Representa- tive images of H&E stained explant cultured P1 testicular sections treated with vehicle (a), various amounts of exogenous FGF4 [10 (b), 25 (c) and 50 (d) ng/mL], FGF receptor inhibitor LY 2,874,455 (LY) [0.1 (e), 0.5 (f) and 1 (g) µM] and co- treatments of various amounts of FGF4 [10 (h), 25 (i) and 50 (j) ng/mL] and 0.5 µM LY for 72 h. Histogram (K) show data of the areas of RT lumen from D1 testicular explants for all treatments (n = 3). Statistical analysis is performed by One- way ANOVA. ***p < 0.001 compared to controls. ST (Seminiferous tubule), RT (Rete testis)

Article Snippet: Recombinant FGF4 (Proteintech, Rosemont, IL, USA) at the final concentrations of 10, 25 and 50 ng/mL, respectively.

Techniques: Inhibition, Staining, Cell Culture

Journal: Journal of cell communication and signaling

Article Title: Dysregulation of Notch-FGF signaling axis in germ cells results in cystic dilation of the rete testis in mice.

doi: 10.1007/s12079-021-00628-0

Figure Lengend Snippet: Fig. 4 Effects of FGF4 and FGF receptor inhibitor on DMRT1 expression in RT cells. Representative DMRT1 immunostain- ing images of neonatal testicular explants treated with vehicle (a), 25 ng/mL FGF4 (b), 0.5 µM LY2874455 (LY, c) and co-treatment of 25 ng/mL FGF4 and 0.5 µM LY (d) for 72 h. The insets are magni- fied images of boxed areas in corresponding pictures. Histogram (e)

Article Snippet: Recombinant FGF4 (Proteintech, Rosemont, IL, USA) at the final concentrations of 10, 25 and 50 ng/mL, respectively.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Myeloid Cell-Specific Lipin-1 Deficiency Stimulates Endocrine Adiponectin-FGF15 Axis and Ameliorates Ethanol-Induced Liver Injury in Mice

doi: 10.1038/srep34117

Figure Lengend Snippet: Wild-type (WT) or mLipin-1KO (KO) mice were pair-fed either a control diet or an ethanol-containing diet for 10 days followed by single gavage of ethanol. ( A ) Representative Western analysis of liver Ac-NF-κB, NF-κB, p-IκBα and IκBα. ( B ) Relative liver protein levels of liver Ac-NF-κB, NF-κB, p-IκBα and IκBα. ( C ) Male C57/6J mice were treated by intraperitoneal injection 1 mg/kg mouse body weight of recombinant FGF19 for six hours. Relative adipose mRNA levels of adiponectin. Relative liver mRNA of Cyp7a1. ( D ) Male intestinal FGF15 knock out (FGF15KO) mice and LOX littermates (WT) mice were fed chow diets. Relative adipose mRNA levels of adiponectin. Relative liver mRNA levels of Cyp7a1. ( E ) Male C57/6J mice were treated by intraperitoneal injection with 0.5 μg/g mouse body weight of recombinant human globular adiponectin (gAcrp) for three days. Relative ileum FGF15. Relative liver mRNA of Cyp7a1. Results are expressed as means ± SEM (n = 4–6 mice). Means without a common letter differ, P < 0.05.

Article Snippet: 12 week old male C57BL/6J mice were treated by intraperitoneal injection with 1 mg/kg mouse body weight of recombinant FGF19 (Gold Biotech, St. Louis, MO) in PBS or vehicle (PBS) and then sacrificed six hours later.

Techniques: Western Blot, Injection, Recombinant, Knock-Out